Category Archives: Research Publications

Publication: Established Models and New Paradigms for Hypoxia-Driven Cancer-Associated Bone Disease

Thomas R. Cox, Jan 2018

Established Models and New Paradigms for Hypoxia-Driven Cancer-Associated Bone Disease

Our new review on the how hypoxia is important in cancer-associated bone disease has just been published in Calcified Tissue International.

Established Models and New Paradigms for Hypoxia-Driven Cancer-Associated Bone Disease

What is Hypoxia and why is it important in cancer?

Continue reading

Publication: The interplay between extracellular matrix remodelling and kinase signalling in cancer progression and metastasis

Thomas R. Cox, Jan 2018

The interplay between extracellular matrix remodelling and kinase signalling in cancer progression and metastasis.

Our recent review discussing the role of the extracellular matrix as a critical regulator of intracellular kinase signalling has just been published in Cell Adhesion & Migration

Extracellular_Matrix_Governs_Kinase_Signalling

Continue reading

Publication: Three-dimensional organotypic matrices from alternative collagen sources as pre-clinical models for cell biology

Thomas R. Cox, Dec 2017

Three-dimensional organotypic matrices from alternative collagen sources as pre-clinical models for cell biology

Our new paper on alternative collagen sources for 3D organotypic cultures for use as pre-clinical models is now out in Scientific Reports

Collaroo: 3D Organotypic matrices for cancer biology

Cellular interactions with the extracellular matrix (ECM) occur in a three-dimensional (3D) context and this essential aspect of the tumour microenvironment can lead to altered sensitivity to therapeutics and even act as a barrier to their delivery. This key feature is often overlooked in pre-clinical studies and is likely one of the central factors contributing to the high attrition rates of lead compounds within the pharmaceutical industry. This organotypic platform allows assessment of lead compounds in both the stromal compartment or in a 3D co-culture setting using large scale collagen preparations from alternative sources.

Continue reading

Matrix: Dissolved – ABC Radio Network Health Report

ABC Radio National LogoThomas R. Cox, Jul 2017

I was recently invited to chat with Norman Swan on ABC Radio National’s Health report about some of the recent work we have been doing on the Extracellular Matrix in cancer.

The extracellular matrix or the matrix is the web- or mesh-like structure that encases the cells in the tissues and organs of our body.

ISDoT - Decellularised Extracellular Matrix

Image: Mayorca-Guiliani AE, Madsen CD, Cox TR et al. Nature Medicine (2017)

We recently developed a new technique which dissolves the cells from tumours to leave behind this matrix, allowing us to study it in unprecedented detail – and we discuss the potential of these research outcomes. Continue reading

Publication: ISDoT – in situ decellularization of tissues for high-resolution imaging and proteomic analysis of native extracellular matrix

Thomas R. Cox, Jun 2017

“We’re seeing things we’ve never seen before”: groundbreaking new technique sheds light on the ‘matrix’ surrounding our cells

Our most recent research  has just been published in Nature Medicine.

In our  paper we describe a new and intuitive new way to dissolve cells from tissues, leaving behind the extracellular matrix (ECM) or ‘matrix’.

The matrix is made up of 100’s of differing building blocks and surrounds the cells in our body. It is incredibly important in the progression and spread of cancer – but up until now it has been notoriously difficult to study in detail.

ISDoT - Decellularised Extracellular Matrix

Image: Mayorca-Guiliani AE, Madsen CD, Cox TR et al. Nature Medicine (2017)

Continue reading

Publication: Transient tissue priming via ROCK inhibition uncouples pancreatic cancer progression, sensitivity to chemotherapy, and metastasis

Thomas R. Cox, Apr 2017

ROCK-ing pancreatic cancer to the core

Our new paper on short-term pulsed treatment, or ‘priming’ as a treatment strategy to boost chemotherapy has just been published in Science Translational Medicine.

Transient tissue priming via ROCK inhibition uncouples pancreatic cancer progression, sensitivity to chemotherapy, and metastasis

The research, spearheaded by Dr. Paul Timpson and Dr. Marina Pajic here at the Garvan Institute of Medical Research in Sydney, has uncovered a promising new approach to treating pancreatic cancer. By targeting the tissue surrounding the tumour to make it ‘softer’, it leads to tumours being more responsive to chemotherapy.

Continue reading

Publication: Pre-metastatic niches: organ-specific homes for metastases

Thomas R. Cox, Mar 2017

Pre-metastatic niches: organ-specific homes for metastases

Our new review on the importance of the pre-metastatic niche (PMN) in cancer has just been published in Nature Reviews Cancer. It summarises the natural progression of pre-metastatic niche formation and evolution, highlighting recent advances and future hurdles.

Premetastatic niches (Nature Reviews Cancer)

What is the pre-metastatic niche (PMN)?

Continue reading

Publication: Relative Stiffness Measurements of Cell-embedded Hydrogels by Shear Rheology in vitro

Thomas R. Cox, Jan 2017

Our new protocol on in vitro shear rheology of cell-embedded hydrogels has just been published in bio-protocol

Cox & Madsen - Bioprotocol

Bio-protocol (ISSN: 2331-8325) is a peer-viewed e-journal established in 2011 by a group of Stanford researchers. Their mission is to make life science research more efficient and reproducible by curating and hosting high quality, open access, life science protocols.

Continue reading

Publication: Tissue Fibrosis and Pancreatic Cancer

Thomas R. Cox, Jun 2016

Fibrosis and Cancer: Partners in Crime or Opposing Forces?

Our recent forum discussing the importance of cancer associated fibrosis in pancreatic cancer has just been published in Trends in Cancer

gr1

Targeting the Extracellular Matrix (ECM) in Pancreatic Ductal Adenocarcinoma (PDAC).

What is cancer associated fibrosis?

Continue reading

Publication: The importance of Lysyl Oxidase (LOX) in Rectal Cancer

Thomas R. Cox, May 2016

In collaboration with the department of oncology and department of clinical and experimental medicine at Linköping University in Sweden, we have just published a new paper looking at the importance of lysyl oxidase (LOX) in rectal cancer patients.

LOX+Rectal2016

Lysyl oxidase immunohistochemistry in rectal cancer samples

Continue reading

Publication: Targeting LOX in Cancer

Thomas R. Cox, Jan 2016

Over the last 2 decades there has been a rapidly increasing number of research papers published (many from our own lab) investigating the function and role of a secreted enzyme called Lysyl Oxidase (LOX). Each paper has added successive small pieces to the complex puzzle of what exactly LOX does in both normal development and human disease, none less so than cancer.

LOX publications by year

The last decade has seen an explosion in the number of papers reporting on Lysyl Oxidase (LOX)

Continue reading

Publication: Dataset for the proteomic inventory and quantitative analysis of the breast cancer hypoxic secretome associated with osteotropism

Thomas R. Cox, Nov 2015

As part of our recent Nature paper (see previous post here) we sought to catalogue all of the different proteins secreted by breast cancer cells (the secretome) under conditions of hypoxia (low oxygen). Hypoxia is a common feature of most solid tumours and is very important in determining how cancer cells behave. Our hypothesis was that the breast cancer secretome changes under hypoxic conditions and that these changes were important in determining the how a patient’s tumour spreads around the body.

To create this catalogue, we performed Mass Spectrometry, a powerful analytical chemistry technique which allows us to both indentify and quantify proteins in a given sample. By creating a list of all the proteins present in the secretome of breast cancer cells, we were able to identify important proteins which may be responsible for determining how and where breast cancer spreads. In our paper we chose to focus specifically on one of these identified secreted proteins called Lysyl Oxidase (LOX).

Open Access – Open Data

Continue reading